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Image Search Results
Journal: Current Biology
Article Title: A Single MicroRNA-Hox Gene Module Controls Equivalent Movements in Biomechanically Distinct Forms of Drosophila
doi: 10.1016/j.cub.2019.06.082
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Software, Microscopy
Journal: Evidence-based Complementary and Alternative Medicine : eCAM
Article Title: Acupuncture Alters Expression of Insulin Signaling Related Molecules and Improves Insulin Resistance in OLETF Rats
doi: 10.1155/2016/9651592
Figure Lengend Snippet: Primers for real-time PCR.
Article Snippet: Nonspecific binding sites were blocked with 5% milk powder diluted in TBS with 0.05% Tween 20 (TBST) for 60 min. Proteins were detected using the following antibodies: rabbit polyclonal antibody for PI3K-p85 (diluted 1 : 3000; Bios; bs-0128R), rabbit polyclonal antibody for phospho-PKC ζ / λ (diluted 1 : 3000; CST; #9378),
Techniques:
Journal: Evidence-based Complementary and Alternative Medicine : eCAM
Article Title: Acupuncture Alters Expression of Insulin Signaling Related Molecules and Improves Insulin Resistance in OLETF Rats
doi: 10.1155/2016/9651592
Figure Lengend Snippet: Effects of acupuncture on mRNA expressions of IRS1, IRS2, PI3K-p85 α , Akt2, aPKC ζ , aPKC λ , and GLUT4 in skeletal muscle of OLETF rats. Acupuncture was applied to the indicated region for 20 min per day, over the course of 21 days. Following administration, the musculus quadriceps femoris was obtained. Relative mRNA expression was determined by real-time RT-PCR. Data are showed as mean ± standard deviation (SD) ( n = 8 each group). ∗∗ P < 0.01 versus SD group; △ P < 0.05 and △△ P < 0.01 versus OLETF group. IRS1: insulin receptor substrate 1; IRS2: insulin receptor substrate 2; PI3K: phosphatidylinositol-3 kinase; PKC: protein kinase C; GLUT4: glucose transporter 4; Akt2: protein kinase B beta.
Article Snippet: Nonspecific binding sites were blocked with 5% milk powder diluted in TBS with 0.05% Tween 20 (TBST) for 60 min. Proteins were detected using the following antibodies: rabbit polyclonal antibody for PI3K-p85 (diluted 1 : 3000; Bios; bs-0128R), rabbit polyclonal antibody for phospho-PKC ζ / λ (diluted 1 : 3000; CST; #9378),
Techniques: Expressing, Quantitative RT-PCR, Standard Deviation
Journal: Evidence-based Complementary and Alternative Medicine : eCAM
Article Title: Acupuncture Alters Expression of Insulin Signaling Related Molecules and Improves Insulin Resistance in OLETF Rats
doi: 10.1155/2016/9651592
Figure Lengend Snippet: Effects of acupuncture on protein expressions of PI3K-p85, phospho-PKC ζ / λ , and GLUT4 in skeletal muscle of OLETF rats. Protein expression was determined by Western blot. GAPDH was used as an internal control. Data are shown as mean ± SD ( n = 8 each group). ∗ P < 0.05 and ∗∗ P < 0.01 versus SD group; △△ P < 0.01 versus OLETF group.
Article Snippet: Nonspecific binding sites were blocked with 5% milk powder diluted in TBS with 0.05% Tween 20 (TBST) for 60 min. Proteins were detected using the following antibodies: rabbit polyclonal antibody for PI3K-p85 (diluted 1 : 3000; Bios; bs-0128R), rabbit polyclonal antibody for phospho-PKC ζ / λ (diluted 1 : 3000; CST; #9378),
Techniques: Expressing, Western Blot, Control